Description
The MC-631 monoclonal antibody reacts with Stage-Specific Embryonic Antigen-3 (SSEA-3), a carbohydrate epitope on the major ganglioside, but not the neutral glycolipid, of mouse embryos and human teratocarcinoma cells. As its name implies, the expression of SSEA-3 is stage-specific and can be used to characterize embryonic cells and monitor their differentiation. However, its expression pattern differs between human and mouse. In the human, SSEA-3 is found on teratocarcinoma (embryonal carcinoma or EC), embryonic inner cell mass (ICM), embryonic stem (ES) cells, and erythrocytes. As human stem cells undergo differentiation, SSEA-3 expression diminishes. In the mouse, SSEA-3 is found on oocytes, ova, zygotes, early cleavage-stage embryos, early blastocysts, ICM, primitive endoderm, and adult kidneys, but not on EC or ES cells.
For Bioimaging, see the protocol at http://www.bdbiosciences.com/support/resources/protocols/ceritifed_reagents.jsp.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
2. An isotype control should be used at the same concentration as the antibody of interest.
3. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
6. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
7. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
8. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
9. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
