BD, 561527, Accutase™ Cell Detachment Solution

To obtain a single-cell suspension: (Use aseptic techniques if you plan on continuing culture of cells.) 1. Wash the cells with PBS at room temperature. 2. Add Accutase™ cell detachment solution to adequately cover the entire area of the culture vessel. 3. Incubate at room temperature for 5 to 10 minutes, or until cells are detached. Cells can also be incubated at 37°C, however enzyme activity will decrease over time. a. Additional time and/or Accutase™ may be needed to disassociate three-dimensional structures. 4. Triturate the cells to aid in obtaining a single-cell suspension. a. Additional cell culture medium may be added to assist in obtaining a single-cell suspension. b. The additional cell culture medium will neutralize the Accutase™ and allow for passaging of cells without additional washes. 5. Remove a small subset of the cell suspension and examine under a microscope to confirm the presence of single cells.
The product should be kept undiluted at -20°C for long term storage, and it may be kept undiluted at 4°C for short term storage.
1. Accutase is a registered trademark of Innovative Cell Technologies, Inc.
2. mTESR™1 is a trademark of StemCell Technologies.
3. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.