BD, 565197, Alexa Fluor® 647 Mouse Anti-GFP 1A12-6-18

Monoclonal antibody 1A12-6-18 reacts with Green Fluorescent Protein (GFP), which is a 27 kDa bioluminescent protein first purified from the jellyfish, Aequorea victoria. GFP exhibits green fluorescence when exposed to blue or ultraviolet light. Additionally, GFP can be introduced into the genome of and expressed in a wide variety of cell types, making it a useful reporter for tracking gene expression or protein localization. A number of GFP mutants have been developed with varying fluorescence intensity and spectra. Monoclonal antibody 1A12-6-18 is known to react with AcGFP, and is not reactive towards ZsGreen1.

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
3. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
4. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
5. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
6. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
8. An isotype control should be used at the same concentration as the antibody of interest.