BD, 565806, BV421 Mouse Anti-Human CD11c 3.9

Name: BV421 Mouse Anti-Human CD11c 3.9
Brand: BD
SKU: 565806

0.1 mg

Catalog Number: 565806

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Category

Cell Cycle Reagents

TagsReagents microscopy-imaging-reagents

CollectionsBD Microscopy & Imaging Reagents for IF/ICC/IHC BD Single-Cell Multiomics Reagents for the BD Rhapsody™ Platform BD Immunoassay Reagents: CBA, ELISA & ELISPOT for Cytokine Panels

Description

The 3.9 monoclonal antibody specifically binds to CD11c, which is also known as Integrin alpha X (αX Integrin/ITGAX), or p150,95 Integrin alpha chain. CD11c is a ~150 kDa type I transmembrane glycoprotein. It is expressed on monocytes, macrophages, granulocytes, NK cells, dendritic cells, and subsets of B and T cells. It associates with CD18 (Integrin beta 2/β2 Integrin) to form the CD11c/CD18 complex, which is also known as p150,95 Integrin, or the Type 4 Complement Receptor (CR4). CD11c/CD18 binds fibrinogen and reportedly serves as a receptor for iC3b and ICAM-1/CD54. CD11c/CD18 functions as an adhesion molecule that mediates cellular binding to ligands expressed on stimulated epithelium and endothelium. The 3.9 monoclonal antibody crossreacts with CD11c expressed by Rhesus macaque leucocytes.
Note: The binding of the 3.9 antibody to CD11c is divalent cation dependent. Therefore, heparin is recommended for use as the blood anticoagulant rather than the EDTA chelating agent that might adversely affect 3.9 antibody binding and cellular staining. For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. An isotype control should be used at the same concentration as the antibody of interest.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
6. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
7. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
8. ProLong® is a registered trademark of Thermo Fisher Scientific, Inc. Waltham, MA.
9. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
10. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
11. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Specifications

General

Source	Mouse SLAMF7, amino acids Ala22-Gly224 (Accession# Q8BHK6.2), with a C-terminal 10 His tag, was expressed in 293E cells.
Molecular Mass	The 213 amino acid recombinant protein has a predicted molecular mass of approximately 23.7 kD. The DTT-reduced and non-reduced protein migrates at approximately 30 – 50 kD by SDS-PAGE. The predicted N-terminal amino acid is Ala.
Purity	> 95%, as determined by Coomassie-stained SDS-PAGE.
Formulation	0.22 µm filtered protein solution is in PBS.
Endotoxin Level	Less than 0.1 EU per µg cytokine as determined by the LAL method.
Concentration	25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling	Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

Ordering

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Unless otherwise stated, the minimum order amount is USD 1,000.
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BD, 565806, BV421 Mouse Anti-Human CD11c 3.9

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