Description
The A1/CD307d monoclonal antibody specifically recognizes CD307d which is also known as Fc receptor-like protein 4 (FCRL4), Fc receptor homolog 4 (FCRH4), or Immune receptor translocation-associated protein 1 (IRTA1). CD307d (FCRL4) is a type I transmembrane glycoprotein that belongs to the IRTA gene family within the Ig gene superfamily. It has four extracellular Ig-like domains, a helical transmembrane region, and two consensus immunoreceptor tyrosine-based inhibition motifs (ITIMs) and one immunoreceptor tyrosine-based switch motif (ITSM) in its cytoplasmic domain. CD307d (FCRL4) binds to aggregated IgA and IgG, and its highest level of expression is on memory B cells in mucosa-associated lymphoid tissues (MALT). Chromosomal abnormalities in the region of the FCRL4 gene are associated with non-Hodgkin lymphoma and multiple myeloma, and CD307d (FCRL4) may play a role in HIV-induced memory B cell dysfunction.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
2. An isotype control should be used at the same concentration as the antibody of interest.
3. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
4. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
5. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
6. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
7. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
9. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
