BD, 566794, Purified Mouse Anti-Human Prominin-1 (CD133) CMab-43

The CMab-43 monoclonal antibody specifically recognizes Prominin-1, which is also known as Prominin (mouse)-like 1 (PROML1 or Prominin-like protein 1), hProminin, Hematopoietic stem cell antigen, Macular dystrophy retinal 2 (MCDR2), Stargardt disease 4 autosomal dominant (STGD4), AC133 antigen, or CD133. Prominin-1 (CD133) is a ~120 kDa five-transmembrane glycoprotein that is encoded by PROM1 (Prominin 1) which belongs to the Prominin gene family. This single-chain, pentaspan transmembrane glycoprotein is comprised of an extracellular N-terminus with two short intracellular sequences and two long extracellular loops followed by an intracellular C-terminus. Prominin-1 (CD133) is expressed on hematopoietic stem cells and progenitor cells found in human bone marrow, cord blood and peripheral blood. It is also expressed on neural stem cells, other tissue-specific stem cells, endothelial progenitor cells, epithelial cells during development, and retinal cells. Prominin-1 (CD133) is likewise detected on some cancer cells from solid tumors, leukemias, cell lines, and can serve as a marker for cancer stem cells. Mutations in PROM1 have reportedly been associated with some retinal diseases.

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. An isotype control should be used at the same concentration as the antibody of interest.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
5. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.