Description
The 2D7 monoclonal antibody selectively recognizes human basophils, Basophils (2D7). The antibody binds to a ~7.2 to 7.5 kDa basophil-specific protein, also known as the 2D7 antigen or ligand, present in secretory granules which has not been conclusively identified. Basophils normally comprise a relatively small percentage (<1%) of total peripheral blood leucocytes. Basophils express high levels of FceRI, a high affinity receptor for IgE. Upon activation through crosslinking of FceRI, basophils degranulate and may thus show reduced staining with the 2D7 antibody due to the release or destruction of the 2D7 antigen. The activated basophils release proinflammatory mediators that can either counter parasitic infections or lead to acute or chronic allergic diseases, including anaphylaxis, asthma, and atopic dermatitis. Activated basophils release a variety of mediators including the vasodilator histamine, proteolytic enzymes, and several cytokines which promote inflammation as well as heparin which prevents blood clotting. The 2D7 monoclonal antibody is useful for identifying human basophils since it does not recognize lymphocytes, monocytes, eosinophils, neutrophils, or mast cells.
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed.
1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
2. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
3. An isotype control should be used at the same concentration as the antibody of interest.
4. Alexa Fluor® 488 fluorochrome emission is collected at the same instrument settings as for fluorescein isothiocyanate (FITC).
5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
7. This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
8. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
9. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
10. Alexa Fluor™ is a trademark of Life Technologies Corporation.
