Description
The HP6002 monoclonal antibody specifically recognizes the subclass of human Immunoglobulin G (IgG) known as IgG2. After human IgG1, human IgG2 is the second most abundant of the four subclasses of IgG found in human serum followed by IgG3 and IgG4. IgG2 is comprised of two identical heavy chains encoded by IGHG2, and two light chains, either Igκ or Igλ, linked by disulfide bonds. The HP6002 antibody binds to the CH2 domain of the IgG2 heavy chain and does not crossreact with other immunoglobulin heavy chain (IgH) subclasses. IgG2 is normally expressed by plasmablasts, plasma cells, and memory B cells as well as by some myeloma or plasmacytoma cells. HP6002 binds to soluble human IgG2, cytophilic IgG2 attached to cells through its Fc region, or human IgG2 antibodies specifically bound to antigens. IgG2 can cross the placenta and disseminate throughout extravascular fluids throughout the body. Human IgG2 serves multiple functions with the transmembrane form serving as an antigen receptor for B lymphocytes and secreted soluble forms participating in various effector functions including antibody-dependent neutralization of toxins or infection by microbes and complement fixation.
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.
1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
2. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
3. An isotype control should be used at the same concentration as the antibody of interest.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
7. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
