Description
The Bcl-2/100 monoclonal antibody specifically recognizes human Bcl-2, also known as B-cell CLL/lymphoma 2. A synthetic peptide corresponding to amino acids 41-54 (GAAPAPGIFSSQPG) of human Bcl-2 was used as the immunogen. This ~26 kDa intracellular protein is encoded by BCL2 (BCL2 apoptosis regulator) which belongs to the Bcl-2 family of regulatory proteins. Bcl-2 is expressed in the endoplasmic reticulum, the outer mitochondrial membrane, and the nuclear envelope. Bcl-2 inhibits apoptosis and is involved in autophagy signaling. It is variably expressed in different cell types including hematopoietic progenitor cells, cells belonging to the T cell and B cells lineages, neurons, epithelial cells, endothelial cells, and melanocyte progenitors. Bcl-2 can form homodimers or heterodimers with other Bcl-2 family members that either block or promote apoptosis. Abnormal expression of Bcl-2 is associated with certain cancers including prostate cancer and B-cell lymphoma.
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
2. Alexa Fluor® 488 fluorochrome emission is collected at the same instrument settings as for fluorescein isothiocyanate (FITC).
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
5. This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
7. Alexa Fluor™ is a trademark of Life Technologies Corporation.
8. An isotype control should be used at the same concentration as the antibody of interest.
9. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
10. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
11. For U.S. patents that may apply, see bd.com/patents.
