BD, 571201, ATAC-Seq Tagmentation and Supplemental Reagents Kit

Name: ATAC-Seq Tagmentation and Supplemental Reagents Kit
Brand: BD
SKU: 571201

1 Each

Catalog Number: 571201

Pricing

Send an RFQ.

View RFQ List

Category

Single Cell Sequencing Reagents

TagsReagents single-cell-multiomics-reagents

CollectionsBD Single-Cell Multiomics Reagents for the BD Rhapsody™ Platform BD Immunoassay Reagents: CBA, ELISA & ELISPOT for Cytokine Panels BD Cell Preparation & Separation Reagents (PBMC, Magnetic, RBC Lysis)

Description

Prior to adding the Tagmentation Buffer to the tagmentation mix, 100% Dimethylformamide (DMF) must be added to the thawed buffer according to the instructions in the library preparation protocols. For detailed instructions on tagmentation step and preparation of standalone ATAC-Seq libraries, refer to Single-Cell ATAC Library Preparation Protocol (Doc ID 23-24473). For detailed instructions on tagmentation step and preparation of multiomic ATAC-Seq and whole transcriptome analysis libraries, refer to Single-Cell ATAC and mRNA Whole Transcriptome Analysis (WTA) Library Preparation Protocol (Doc ID 23-24474). For detailed instructions on how to use the BD Rhapsody™ Single-Cell Analysis system, refer to the BD Rhapsody™ HT Xpress Single-Cell Analysis System Instrument User Guide (Doc ID 23-24257) or the BD Rhapsody™ HT Xpress System Instrument User Guide for Scanner-free Workflow (Doc ID 23-24256). For sample preparation prior to the ATAC-Seq assay, refer to BD Rhapsody™ System Preparing Single Cell Suspensions Protocol (Doc ID 23-24126). For information on the bioinformatic pipeline for the ATAC-Seq assay, refer to BD® Single-Cell Multiomics Bioinformatics Handbook (Doc ID 54169). For safety information, see the BD Rhapsody ™ Single-Cell Analysis System Instrument User Guide (Doc ID 23-24257). Use standard laboratory safety protocols. Read and understand the safety data sheets (SDSs) before handling chemicals. To obtain SDSs, go to regdocs.bd.com or contact BD Biosciences technical support at [email protected]. Warning: All biological specimens and materials coming into contact with them are considered biohazardous. Handle as if capable of transmitting infection and dispose of with proper precautions in accordance with federal, state, and local regulations. Never pipette by mouth. Wear suitable protective clothing, eyewear, and gloves. Warning: 571201 ATAC-Seq Tagmentation Kit, Kit component 51-9023085 Digitonin. Digitonin contains 2 wt.% Digitonin, CAS number 11024-24-1, EC number 234-255-6, and 98 wt.% Dimethyl sulfoxide, CAS number 67-68-5, EC number 200-664-3. These reagents are classified as hazardous according to the Globally Harmonized System of Classification and Labelling of Chemicals (GHS). Warning Combustible liquid. Harmful in contact with skin. May cause damage to organs through prolonged or repeated exposure. Prevention Keep away from open flames/hot surfaces. - No smoking. Do not breathe dust/fume/gas/mist/vapors/spray. Wear protective gloves/protective clothing/eye protection/face protection. Response IF ON SKIN: Wash with plenty of soap and water. Call a POISON CENTER/doctor if you feel unwell. Get medical advice/attention if you feel unwell. Take off contaminated clothing and wash it before reuse. In case of fire: Use water spray to extinguish. In case of fire: Use carbon dioxide to extinguish. Storage Store in a well-ventilated place. Keep cool. Disposal Dispose of contents/container to an approved facility in accordance with local, regional, national, and international regulations. Warning: 571201 ATAC-Seq Tagmentation Kit, Kit component 51-9022689 Proteinase K Danger: Proteinase K contains 2.53 wt.% Proteinase, Tritirachium album serine, CAS number 39450-01-6, EC number 254-457-8. These reagents are classified as hazardous according to the Globally Harmonized System of Classification and Labelling of Chemicals (GHS). Danger May cause allergy or asthma symptoms or breathing difficulties if inhaled. Prevention Avoid breathing dust/fume/gas/mist/vapors/spray. [In case of inadequate ventilation] wear respiratory protection. Response IF INHALED: Remove person to fresh air and keep comfortable for breathing. If experiencing respiratory symptoms: Call a POISON CENTER or doctor/ physician. Disposal Dispose of contents/container to an approved facility in accordance with local, regional, national, and international regulations.

1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
4. For U.S. patents that may apply, see bd.com/patents.

Specifications

General

Brand	BD Rhapsody™
Reactivity	Human (Tested in Development)
Application	Single cell multiomics experiments on the BD Rhapsody™ platform (Tested During Development)
Regulatory Status	RUO

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:

[email protected]

Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.