Description
ErbB2 (Neu or Her-2) is a member of the erbB family of growth factor receptors. These factors possess constitutive tyrosine kinase activity and are commonly overexpressed in breast and ovarian carcinomas. While other erbB family members' ligands, such a EGF and NDF, are well characterized, a natural ligand for erbB2 remains unknown. ErbB2 forms heterodimers with erbB1/EGFR, erbB3, and erbB4, and can modulate their ligand affinities. Thus, erbB2 alters the intracellular responses elicited by EGF and NDF. This control is due to the fact that erbB2, when in complex with another erbB family receptor, decelerates the rate of ligand dissociation. Therefore, erbB2 may act as a signaling subunit for other receptors rather than a true growth factor receptor. Due to high sequence homology, this antibody may cross-react with the 180 kDa EGFR.
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
