Description
Integrins are membrane receptors that mediate cell-cell or cell-matrix adhesion. They are transmembrane heterodimers composed of α and β subunits and are connected to the cytoskeleton. At least 20 integrins, formed from combinations of 12 α and 9 β subunits, have been reported. Many of these have been implicated as transducers of molecular signals. The integrin α5 subunit is 1049 amino acids with a molecular weight of 150-165 kDa. α5 and β1 form a functional fibronectin receptor which is important for vascular development in mice. Both subunits of the fibronectin receptor are heavily glycosylated in their extracellular domains. This modification is essential for proper cell attachment to basal membranes. Integrin α5 has been reported to suppress apoptosis by a Bcl-2 pathway and the C-terminal region is critical for cell motility and cytoskeletal rearrangements.
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
