Description
The 4G2 monoclonal antibody specifically recognizes CD319 which is also known as CD2-like receptor activating cytotoxic cells (CRACC), Signaling lymphocytic activation molecule 7 (Slamf7), or novel Ly9. CD319 (CRACC) is a single-pass type I transmembrane glycoprotein that is encoded by Slamf7 (SLAM family member 7) which belongs to the SLAM family within the CD2-subset of the immunoglobulin superfamily (IgSF) of cell surface receptors. This receptor is comprised of an extracellular region with one N-terminal Ig-like V-type (IgV) domain followed by one Ig-like C2-type (IgC2) domain. Its cytoplasmic tail contains two immunoreceptor tyrosine-based switch motifs (ITSMs). This receptor is variably expressed on activated CD4+ T cells and CD8+ T cells, NKT cells, NK cells, B cells, monocytes, macrophages, and dendritic cells. CD319 (CRACC) serves as a homotypic adhesion molecule that can self-associate and trigger cellular activation, such as cell-mediated cytotoxicity, when its cytoplasmic domains are associated with adaptor molecules including EWS-activated transcript 2 (EAT-2) and possibly EAT-2-related transducer (ERT). In cells that lack EAT-2, CD319 (CRACC) may exhibit an inhibitory function.
Put all BD® AbSeq Reagents to be pooled into a Latch Rack for 500 µL Tubes (Thermo Fisher Scientific Cat. No. 4900). Arrange the tubes so that they can be easily uncapped and re-capped with an 8-Channel Screw Cap Tube Capper (Thermo Fisher Scientific Cat. No. 4105MAT) and the reagents aliquoted with a multi-channel pipette. BD® AbSeq tubes should be centrifuged for ≥ 30 seconds at 400 × g to ensure removal of any content in the cap/tube threads prior to the first opening.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography and conjugated to BD® AbSeq oligonucleotide under optimal conditions.
1. This reagent has been pre-diluted for use at the recommended volume per test. Typical use is 2 µl for 1 × 10^6 cells in a 200-µl staining reaction.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
5. Illumina is a trademark of Illumina, Inc.
6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
7. Please refer to bd.com/genomics-resources for technical protocols.
8. For U.S. patents that may apply, see bd.com/patents.
