Description
Use Native Sample Buffer to retain native protein structure and mass-to-charge ratios during protein electrophoresis. This sample buffer is nondenaturing, containing no SDS, and has no reducing agent. The mobility of proteins in native gels depends on a number of factors in addition to molecular weight, including protein shape and charge.
• The biological activity of most proteins is retained
• Some protein-protein interactions may be maintained
• Native gel electrophoresis cannot be used for molecular weight estimation
• Since migration is influenced by structure, proteins of the same size can often be easily resolved
• Dry samples can be dissolved directly into the sample buffer
Precast gels that can be used for native electrophoresis
Note: Most proteins have an acidic or slightly basic pI (~3–8) and are run with the power supply connected to the electrophoresis chamber as for SDS-PAGE. If more basic proteins (pl >8.5) are of interest, change the polarity of the electrodes, since they have a net positive charge.
Related Products
• 10x Tris/Glycine ( 1610734 )
Related Categories
• Running Buffers and Reagents
• Sample Loading Buffers and Reagents
• Electrophoresis Chambers
• Power Supplies
• Protein Stains
• Gel Imaging Systems
Supporting Documentation
• Native Sample Buffer Instruction Manual
• Mini-PROTEAN Precast Gels Instruction Manual and Application Guide
• Criterion Gel Application Guide Instruction Manual
Online Resources
• Protein Electrophoresis
• Western Blotting
• Imaging and Analysis
