Description
Human Anti-Benralizumab Antibody, clone AbD59973 is a paratope specific, inhibitory, anti-idiotypic antibody (Type 1) that specifically recognizes the free human monoclonal antibody drug benralizumab. It does not recognize the drug target, human interleukin 5 receptor α (IL5Rα), nor benralizumab in complex with IL5Rα. This antibody can be used to measure free benralizumab levels in serum from patients. Clone AbD59973 is available as a monovalent Fab antibody incorporating a SpyTag at the C-terminus end of the antibody heavy chain for conversion by the end user into alternative formats using any of the SpyCatchers available in our catalog under product code TZA069 . It is also available as a bivalent Fab antibody already conjugated to HRP via coupling to the HRP conjugated BiSpyCatcher2 ( TZC002P ) for use in direct ELISA under product code TZA069P . A pair of anti-benralizumab antibodies can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug; clone AbD59973ad is recommended as a detection antibody, paired with Human Anti-Benralizumab Antibody, clone AbD60095ad ( TZA068 ) as the capture reagent. Benralizumab (trade name Fasenra) is a humanized monoclonal antibody of the IgG1/kappa isotype that binds with high affinity to a conformationally distinct epitope within domain 1°F the α-chain of IL-5R, and thus inhibits proliferation of IL-5-dependent cell lines, and otherwise blocks signaling through the IL-5 receptor. Benralizumab is indicated as a maintenance treatment for severe asthma and an eosinophilic phenotype. View a summary of all anti-belimumab antibodies
Storage Information
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
