Cytiva, 11000735, Filter 280 nm

Name: Filter 280 nm
Brand: Cytiva
SKU: 11000735

Catalog Number: 11000735

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Category

TagsChromatography products Chromatography equipment and accessories UV pH and conductivity monitors for chromatography

CollectionsCytiva Chromatography Columns, Resins & Lab Glassware Cytiva Protein Analysis Equipment & Supplies Collection

Description

Monitor UVis-920 is a high-precision, multipurpose, standalone UV monitor. The use of different filters allows the monitor to cover wavelengths from 200 nm to 700 nm, without changing the lamp. Pre-assembled filters with holders are placed with a push-button mechanism. Other wavelengths can be achieved by using an appropriate filter assembled in an empty filter holder. The fiber optics together with flow cell configurations cover a wide flow range for a variety of applications whilst providing excellent flow cell accessibility. This allows columns to be placed close to the flow cell, which reduces band broadening and gives a high signal-to-noise ratio. This together with a low noise and a low thermal drift will allow a sensitive, stable and reliable UV/Vis absorbance detection

FAQs

Question: Which wavelength does the UVis-920 optical filter select?

Answer:280 nm, commonly used for UV absorbance monitoring of proteins.

Question: Is this a sample filtration membrane?

Answer:No. It is an optical wavelength filter installed in a UV/Vis monitor.

Question: Does the 280 nm filter include the full detector?

Answer:No. The optical filter is an accessory for a compatible UVis-920 monitor and works with its existing detector and flow-cell components.

Application Note

The Cytiva 280 nm UV filter is a wavelength-selective optical component for the UVis-920 UV/Vis monitor used in protein chromatography. It selects the 280 nm detection wavelength commonly used to follow absorbance from aromatic amino acid residues in proteins as material passes through a chromatography flow cell. The filter is supplied as a monitor accessory, not as a sample filter membrane, liquid filter cartridge or standalone detector. The UVis-920 uses interchangeable preassembled optical filters to select measurement wavelengths without replacing the instrument lamp. Choosing 280 nm allows protein elution peaks to be monitored under suitable buffer conditions, although absorbance also depends on the target molecule and any UV-absorbing components in the mobile phase. Filter handling should protect the optical surfaces from scratches and contamination, and the correct holder and instrument fitting should be used. This wavelength differs from 260 nm nucleic acid monitoring and other available filter options. The component does not include the flow cell or monitor electronics.

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Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
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