Cytiva, 11003275, HiTrap Capto MMC, 5 × 5 mL

The multimodal functionality gives a different selectivity compared with traditional ion exchangers including binding of proteins regardless of ionic strength of the loading material . This means that the resin may be used for direct load of clarified feedstocks, without prior dilution to reduce the conductivity of the starting material. To reduce tedious sample preparation, the top filter in HiTrap™ Capto MMC has been optimized for loading sonicated unclarified cell lysate directly on the column. The Capto™ MMC ligand may interact with proteins in many different ways. It contains a carboxyl group, and thus it is a weak cation exchanger. However, in addition to the ionic interactions several other types of interactions are involved, including hydrogen bonding and hydrophobic interaction.