Cytiva, 17005001, Sephadex G-75, 100 g

Name: Sephadex G-75, 100 g
Brand: Cytiva
SKU: 17005001

Catalog Number: 17005001

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Category

Process-Scale Chromatography Resins

Pack Size100 g

TagsChromatography resins Size exclusion resins Molecular and Immunodiagnostics Chromatography products RNA and DNA purification columns resin

CollectionsCytiva Chromatography Columns, Resins & Lab Glassware Cytiva Protein Analysis Equipment & Supplies Collection

Description

Sephadex G-75 is well established gel filtration resin for desalting and buffer exchange of large biomolecules >80 000 molecular weight.
Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
Suitable for buffer exchange and cleanup of large biomolecules, >80 000 molecular weight
Highest exclusion limit, 80 000, in the Sephadex G-range
Classic gel filtration resin.
Sephadex is a gel filtration resin prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range.

Specifications

General

Autoclavable	In wet form (pH 7.0) at 120°C for 30 min
Exclusion limit (M r ) (globular proteins)	> 8 x 10⁴
Fractionation range (M r ) globular proteins	4 × 10³–5 × 10⁴
Matrix	Cross-linked dextran
PSD dry (within specification)	40 µm – 120 µm
PSD wet	92 µm – 293 µm (40 µm – 320 µm)
Recommended maximum operational flow velocity	90 cm/h in a BPG 100/500 column with 10 cm inner diameter and 20 cm bed height (at room temperature using water)
Storage	4 to 30°C
Swelling of dry resin	19 mL/g
pH stability, operational	2–10

Ordering

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