Cytiva, 17043003, CNBr-Activated Sepharose 4B, 1 kg

Name: CNBr-Activated Sepharose 4B, 1 kg
Brand: Cytiva
SKU: 17043003

Catalog Number: 17043003

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Category

Process-Scale Chromatography Resins

Pack Size1 kg

TagsChromatography resins Chromatography products Affinity resins - specific groups resin

CollectionsCytiva Chromatography Columns, Resins & Lab Glassware Cytiva Protein Analysis Equipment & Supplies Collection

Description

CNBr-activated Sepharose 4B is a pre-activated resin used for coupling antibodies or other large proteins containing -NH₂ groups to the Sepharose media, by the cyanogen bromide method, without an intermediate spacer arm.
For immobilizing antibodies or other large proteins containing -NH₂ groups by coupling them to the matrix
Over 25 years proven track record for coupling ligands containing primary amino groups
Multipoint attachment resulting in a chemically stable product
The coupling reaction by the cyanogen bromide method is spontaneous, rapid and easy to carry out
CNBr-activated Sepharose 4B is a pre-activated resin for immobilization of ligands containing primary amines. It provides a very convenient way to immobilize ligands by the cyanogen bromide method. The coupling reaction is spontaneous, rapid and easy to carry out. The application area covers immobilization of proteins, peptides and nucleic acids.

Specifications

General

Chromatography technique	Affinity coupling
Certificate of Analysis	Yes
Chemical stability	Stable to commonly used aqueous solutions. Can be used with non-ionic detergents, denaturing solvents, e.g. 8 M urea and 6 M guanidine hydrochloride. Stable in organic solvents, such as 50% dimethylformamide and 50% dioxane
Group To be Coupled	-NH₂
Matrix	4% agarose
Particle Size	45 μm-165 μm
Particle size, d 50V	~90 µm
Pressure/Flow Specification	base matrix 70-140 cm/h, pressure drop cm H₂O/bed height=15 cm, bed height 10 cm, 5 cm i.d.
Storage	2 to 8°C

FAQs

Question: What is the purpose of CNBr activation?

Answer:It enables covalent immobilization of suitable amine-containing ligands on the agarose matrix.

Question: What quantity is supplied?

Answer:1 kg of freeze-dried activated Sepharose 4B.

Question: Is a binding ligand already immobilized on the resin?

Answer:No. The activated matrix is designed for user-defined ligand coupling.

Application Note

Cytiva CNBr-Activated Sepharose 4B is a preactivated agarose chromatography matrix for preparing custom affinity adsorbents. Cyanogen bromide activation creates reactive sites that enable covalent attachment of suitable proteins, peptides, antibodies, and other ligands containing primary amino groups. Researchers can immobilize their selected ligand and develop selective purification or target-capture procedures using the resulting affinity medium. The 1 kg freeze-dried package provides bulk activated Sepharose 4B for coupling and column packing at laboratory or preparative scales. Before use, the medium is swollen and washed under the specified coupling conditions, followed by ligand immobilization and blocking of residual reactive groups. It is a coupling support rather than an antibody-conjugated prepacked column or a finished affinity resin with a fixed target specificity.

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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