Cytiva, 17051004, Q Sepharose Fast Flow, 5 L
A strong anion exchanger based on the well established Sepharose™ Fast Flow ion exchange platform
Catalog Number: 17051004
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Pack Size5 L
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| BioProcess resin | Yes |
|---|---|
| Certificate of Analysis | Yes |
| Chemical stability | Stable to commonly used aqueous buffers:1.0 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol |
| Exclusion limit (M r ) (globular proteins) | ~4 × 10⁶ |
| Ion Exchanger Type | Strong anion exchanger |
| Ionic Capacity | 0.18-0.24 mmol Cl - /ml medium |
| Ligand | Quaternary amine |
| Matrix | cross-linked, 6% agarose particles |
| Particle size, d 50V | ~90 µm |
| Pressure/Flow Specification | 400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm |
| Storage | 4 to 30°C, 20% Ethanol |
| pH ligand fully charged | Entire pH range |
| pH stability, CIP | 2–14 |
| pH stability, operational | 2–12 |
Answer:It is a strong anion exchanger with quaternary ammonium functional groups.
Answer:This product contains 5 L of bulk chromatography medium.
Answer:No. The medium is supplied as bulk resin for column packing.
Q Sepharose™ Fast Flow is a strong anion exchange chromatography medium used for charge-based purification of proteins, biomolecules, and selected process impurities. Its quaternary ammonium functional groups maintain a positive charge over a broad working pH range, allowing negatively charged species to bind under suitable buffer conditions. The cross-linked agarose matrix is designed for robust process flow and is widely used for downstream capture, intermediate purification, and polishing operations. This configuration supplies 5 L of bulk resin intended for packing process chromatography columns. Binding behavior depends on sample pH, ionic strength, target charge, and loading conditions. The medium can be incorporated into a qualified manufacturing workflow with appropriate buffer selection, column packing, cleaning, and lifetime evaluation. This is not a prepacked chromatography column.
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For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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