Cytiva, 17072960, SP Sepharose Fast Flow cation exchange resin, 60 L

Name: SP Sepharose Fast Flow cation exchange resin, 60 L
Brand: Cytiva
SKU: 17072960

Well-proven strong cation exchange chromatography resin for large-scale bioprocess separations

Catalog Number: 17072960

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Category

Process-Scale Chromatography Resins

Pack Size60 L

TagsChromatography resins Ion exchange resins Chromatography products resin

CollectionsCytiva Chromatography Columns, Resins & Lab Glassware Cytiva Protein Analysis Equipment & Supplies Collection

Description

SP Sepharose Fast Flow is a sulphopropyl (SP) strong cation exchange chromatography resin for fast protein purification.
Well established strong cation exchanger.
Based on Sepharose Fast Flow ion exchange resin.
High chemical stability enables proven CIP and sanitization protocols.
Hydrophilic base matrix ensures low levels of nonspecific binding and low levels of host cell-derived impurities in the elution pool.
Fulfills industrial demands for security of supply, robust performance, and regulatory support.
Streamlined scale-up
SP Sepharose Fast Flow uses the Sepharose Fast Flow ion exchange resin, which is well established in industrial downstream processing. The highly crosslinked agarose resin enables high flow rates and high productivity.
Composed of crosslinked 6% agarose beads with sulphopropyl (SP) strong cation exchange groups, this resin has high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols.
Scale-up with these SP media (resins) is straightforward. Scale-up is further aided by a range of prepacked process development tools, for example PreDictor 96-well plates, PreDictor RoboColumns™, and HiTrap columns.
For even faster flow rates and better binding capacity in scale-up, Capto S and other Capto resins are a good option.
Successful use – research scale
SP Sepharose Fast Flow media (resins) are used in a variety of research applications, including:
Two-step ion exchange chromatography of human calprotectin from neutrophils for structural studies
Purification of APT1 from rat liver cytosol
Purification of a manganese peroxidase from Panaeolus sphinctrinus
Compatible empty columns – research scale
Cytiva's solutions include a variety of empty columns for research scale. SP Sepharose Fast Flow is compatible with two of these lines, Tricorn and XK columns.
Compatible empty columns – bioprocess scale
For process-scale applications, Cytiva recommends AxiChrom columns. SP Sepharose Fast Flow can also be packed in the other column platforms such as BPG and Chromaflow.
Prepacked columns – research scale
This strong cation exchange resin is available in the following prepacked formats:
HiTrap SP FF columns – convenient for easy use with a syringe, peristaltic pump, or chromatography system such as ÄKTA system
HiScreen SP FF columns for scale up and process development.
HiPrep SP FF columns for convenient scale-up.
Prepacked columns – bioprocess scale
Prepacked ReadyToProcess columns are available for bioprocess applications.

Specifications

General

BioProcess resin	Yes
Certificate of Analysis	Yes
Chemical stability	Stable to commonly used aqueous buffers:1.0 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol
Exclusion limit (M r ) (globular proteins)	~4 × 10⁶
Ion Exchanger Type	Strong cation exchanger
Ionic Capacity	0.18-0.25 mmol H + /ml medium
Ligand	Sulphopropyl
Matrix	cross-linked agarose, 6%, spherical
Particle Size	45 µm-165 µm
Particle size, d 50V	~90 µm
Pressure/Flow Specification	400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm
Storage	4 to 30°C, 20% Ethanol + 0.2 M Sodium Acetate
pH ligand fully charged	Entire pH range
pH stability, CIP	3–14
pH stability, operational	4–13

FAQs

Question: What ion-exchange functionality does this resin use?

Answer:It is a strong cation exchanger with sulfopropyl groups on a cross-linked agarose matrix.

Question: What is the pack volume?

Answer:The selected bulk resin pack contains 60 L and is listed as available on request.

Question: Is the material supplied as a prepacked column?

Answer:No. It is bulk chromatography resin that must be packed and qualified in a suitable column.

Application Note

Cytiva SP Sepharose Fast Flow is a strong cation-exchange chromatography resin for protein capture and intermediate purification at process scale. Sulfopropyl functional groups are coupled to a spherical, cross-linked 6% agarose matrix, enabling binding of appropriately charged target proteins under controlled buffer conditions. The 60 L bulk pack supports large column packing and process-development transfer to manufacturing-scale chromatography. Average bead diameter is approximately 90 µm, providing a balance of pressure-flow properties and binding performance for packed-bed operation. Select the loading pH and conductivity based on the protein's charge behavior and impurity profile, and establish a suitable equilibration, wash, elution and cleaning cycle. Packed-bed performance depends on column geometry, sample viscosity and packing quality. The medium is a chromatography resin, not a prepacked column or a single-use filter.

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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