Cytiva, 17097305, Phenyl Sepharose 6 Fast Flow (High Sub), 200 mL
Catalog Number: 17097305
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Category
Pack Size200 mL
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| BioProcess resin | Yes |
|---|---|
| Certificate of Analysis | Yes |
| Chemical stability | Stable in commonly used aqueous buffers - 1.0 M NaOH, 3 M Ammonium sulphate, 30% isopropanol, 70% ethanol, 10% ethylene glycol, 0.5% SDS, 6 M guanidine-hydrochloride, 8 M Urea |
| Ligand | Phenyl |
| Ligand Concentration | ~45 µmol phenyl/mL resin |
| Matrix | cross-linked agarose, 6%, spherical |
| Particle Size | 45 µm-165 µm |
| Particle size, d 50V | ~90 µm |
| Pressure/flow characteristics | 250-400 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20⁰C using buffers with the same viscosity as water). |
| Storage | 4 to 30°C, 20% Ethanol |
| pH stability, CIP | 2–14 |
| pH stability, operational | 3–13 |
Answer:It identifies the high phenyl-ligand substitution version of this hydrophobic interaction chromatography resin.
Answer:By reducing salt concentration to weaken the hydrophobic interaction.
Answer:No. It is 200 mL of bulk Phenyl Sepharose 6 Fast Flow resin for column packing.
Phenyl Sepharose™ 6 Fast Flow (High Sub) is a hydrophobic interaction chromatography resin used to separate proteins by differences in surface hydrophobicity. Phenyl ligands attached to the Sepharose 6 Fast Flow matrix form reversible hydrophobic interactions with target molecules, commonly under elevated salt conditions. The high-substitution variant provides a higher phenyl-ligand density than the low-substitution grade and can be useful when stronger hydrophobic interaction is required. Samples are generally conditioned with an appropriate salt concentration before loading. A decreasing-salt gradient then weakens binding and permits elution of proteins with different hydrophobic characteristics. Screening salt type, starting concentration, pH and residence time is essential because retention and recovery vary widely between proteins. Monitor aggregation and loss of activity during method development, especially for sensitive biological products. The resin is supplied as 200 mL of bulk medium for packing chromatography columns and is not a prepacked cartridge or ready-to-use spin column.
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