Cytiva, 17098103, CNBr-Activated Sepharose 4 Fast Flow, 250 g

CNBr-Activated Sepharose 4 Fast Flow is used to prepare customized affinity chromatography media by coupling large ligands that contain primary amino groups. The preactivated resin supports rapid, efficient coupling without specialized activation equipment, making it suitable for developing biospecific purification steps and scaling them into industrial bioprocesses. Its matrix consists of cross-linked 4% agarose beads preactivated with cyanogen bromide. This chemistry permits multipoint attachment of protein ligands, which helps minimize ligand leakage during use. The 17098103 configuration supplies 250 g of freeze-dried resin stabilized with additives. A typical coupling workflow starts by swelling and washing the gel, proceeds to spontaneous ligand coupling, and then uses the resulting medium in an affinity column. As a BioProcess resin, it supports industrial manufacturing and use in established approved processes. The resin can also be regenerated and cleaned in place. Regular cleaning-in-place removes lipids, precipitates, and denatured proteins that may accumulate when processing crude feed, helping maintain capacity, flow properties, and general chromatographic performance. The Sepharose Fast Flow base provides a familiar platform for process development, while multiple bulk pack sizes support scale-up. This combination of preactivation, agarose construction, efficient amino-ligand coupling, reduced ligand leakage, and process support makes the product well suited to laboratories and manufacturers building robust custom affinity purification media.