Cytiva, 17098105, CNBr-Activated Sepharose 4 Fast Flow, 2 kg
Fast, effective cyanogen bromide-activated resin for high-performance coupling of large amino-containing ligands.
Catalog Number: 17098105
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Category
Pack Size2 kg
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| Chromatography technique | Affinity coupling |
|---|---|
| BioProcess resin | Yes |
| Certificate of Analysis | Yes |
| Chemical stability | Stable in commonly used aqueous buffers provided that the ligand withstand the pH or the chemical environment |
| Group To be Coupled | Primary amines |
| Ligand | Cyanogen bromide activated |
| Matrix | 4% cross-linked agarose |
| Particle Size | 45 µm-165 µm |
| Particle size, d 50V | ~90 µm |
| Pressure/Flow Specification | Base matrix: 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm |
| Storage | Unsused: 2 to 8°C - Used: 2 to 8°C in 20% Ethanol |
| pH stability, CIP | 2–11 (ligand dependent) |
| pH stability, operational | 3–11 |
Answer:This configuration contains 2 kg of freeze-dried activated resin.
Answer:The resin uses highly cross-linked 4% agarose with a mean particle size of about 90 µm.
Answer:It is used to covalently couple custom ligands for affinity chromatography.
CNBr-Activated Sepharose™ 4 Fast Flow is a preactivated agarose resin for covalent immobilization of ligands containing primary amino groups or related nucleophiles. This configuration contains 2 kg of freeze-dried material. The matrix is highly cross-linked 4% agarose with a mean particle size of approximately 90 µm and a particle-size range of 45–165 µm. After swelling, one gram of dry material yields approximately 4–5 mL of drained gel. The resin is used to prepare custom affinity media for purification of antibodies, antigens, enzymes, binding proteins, and other biomolecules. Its Fast Flow matrix provides higher mechanical strength than traditional Sepharose 4B for scale-up and larger packed columns.
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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