Cytiva, 17108704, SP Sepharose High Performance, 5 L
Catalog Number: 17108704
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Category
Pack Size5 L
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| BioProcess resin | Yes |
|---|---|
| Certificate of Analysis | Yes |
| Charged Groups | - SO₃ - |
| Chemical stability | Stable in commonly used buffers: 8 M urea, 6 M guanidine HCl, 70% ethanol, 1 M NaOH,* 1 M acetic acid, 30% isopropanol, 30% acetonitrile, 2% SDS |
| Exclusion limit (M r ) (globular proteins) | ~4 × 10⁶ |
| Ion Exchanger Type | Strong cation exchanger |
| Ionic Capacity | 0.15-0.20 mmol H + /ml |
| Ligand | Sulphopropyl |
| Ligand Coupling Method | Ether linkages |
| Matrix | 6% cross-linked agarose |
| Particle Size | 24 µm-44 µm |
| Particle size, d 50V | ~34 µm |
| Pressure/Flow Specification | 150 cm/h (at < 3 bar, 25°C, 10 cm bed height) |
| Storage | 4 to 30°C, 20% Ethanol + 0.2 M Sodium Acetate |
| pH stability, CIP | 3–14 |
| pH stability, operational | 4–13 |
Answer:It is a strong cation exchange resin. The sulfopropyl ligand binds positively charged proteins under suitable pH and conductivity conditions.
Answer:The cross-linked agarose beads have a mean size of approximately 34 µm. This smaller bead size supports high-resolution charge-based separations.
Answer:No. It contains 5 L of bulk chromatography resin for packing suitable process or laboratory columns. Column hardware, connectors, and operating systems are separate.
Cytiva SP Sepharose High Performance is a strong cation exchange resin for high-resolution intermediate and final purification of proteins. Its sulfopropyl (SP) ligand carries a negatively charged sulfonate group that captures positively charged biomolecules at suitable buffer pH and ionic strength. The rigid, highly cross-linked 6% agarose matrix has a mean bead size of approximately 34 µm, giving a high surface area for biomolecular binding while supporting reproducible chromatographic separations. Supplied in a 5 L bulk-resin pack, the material is intended for packing compatible chromatography columns, not for direct use as a disposable prepacked cartridge. It is especially suited to polishing applications where separation of closely related charge variants matters. Elution can be controlled using salt gradients or pH changes. The typical long-term pH stability range is 4–13, with broader limits for brief cleaning steps. Store in the specified preservative solution and maintain appropriate column-packing and sanitization protocols.
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