Cytiva, 17524801, HisTrap HP column, 1 x 5 ml

Name: HisTrap HP column, 1 x 5 ml
Brand: Cytiva
SKU: 17524801

Prepacked nickel charged column for maximized recovery of histidine-tagged proteins

Catalog Number: 17524801

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Category

Protein Purification Columns

Pack Size1 x 5 mL

TagsPrepacked columns Chromatography products Affinity tagged protein

CollectionsCytiva Chromatography Columns, Resins & Lab Glassware Cytiva Protein Analysis Equipment & Supplies Collection

Description

Ni Sepharose High Performance columns His tag protein purification by IMAC is both popular and highly efficient. HisTrap HP columns are packed with Ni Sepharose High Performance (HP) affinity resin. This resin consists of highly cross-linked agarose beads to which a chelating group has been coupled. The chelating group is precharged with nickel, which selectively retains proteins with exposed histidine groups. HisTrap HP IMAC resin has a binding capacity that exceeds 40 mg/mL, resulting in high protein recovery. The small bead size (34 µm) contributes to a purer purification compared to nickel resins of larger bead size. Successful use of HisTrap HP protein purification columns in published research HisTrap HP columns are used in a variety of applications, including: purification of His tagged membrane proteins to study lipid binding purification of His tagged spider toxin and variants to evaluate sodium channel inhibition Other Cytiva products for His tag protein purification Cytiva provides a large variety of Life Sciences solutions for IMAC protein purification. For high binding capacity or when scalability is needed, consider HisTrap FF Crude columns.

FAQs

Question: How much affinity medium is in this column?

Answer:The prepacked bed contains 5 mL of Ni Sepharose High Performance resin.

Question: What bed dimensions and recommended flow rate apply?

Answer:The bed is approximately 16 × 25 mm with a recommended flow rate of 5 mL/min.

Question: How does the 5 mL format compare with the 1 mL version?

Answer:It has five times the resin volume, supporting greater total protein loading when binding conditions are comparable.

Application Note

Cytiva HisTrap HP 5 mL affinity columns provide a larger prepacked bed for preparative purification of polyhistidine-tagged proteins. Each column contains 5 mL of Ni Sepharose High Performance resin, a nickel-charged, highly cross-linked agarose medium with an average particle size of 34 µm. The bed measures approximately 16 × 25 mm and supports a recommended flow rate of 5 mL/min. At least 40 mg of His-tagged protein can bind per mL of medium under suitable conditions, making the larger bed useful when load mass exceeds the capacity of a 1 mL format. Apply clarified sample in an appropriate binding buffer, reduce nonspecific association with a controlled washing step, and recover target protein with a suitable imidazole-containing elution buffer. The prepacked format can connect to compatible syringe, pump and chromatography systems. Monitor pressure and flow, avoid exposing the resin to incompatible solutions, and maintain the prescribed storage medium between runs.

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