Cytiva, 17528601, HisTrap FF Crude column, 5 x 5 ml

His-tag protein purification from unclarified lysates HisTrap FF crude is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow. After thorough cell disruption, it is possible to load the unclarified lysate on the column, without precentrifugation and filtration. If you plan to load unclarified samples, it is important to ensure that lysis is as complete as possible. We recommend adding lysozyme and DNase I, followed by mechanical lysis (e.g., by sonication) for a longer duration than typically performed. Tips and tricks for using these IMAC columns Interested in maximizing the performance of your column? Watch Cytiva's HisTrap FF crude column video to get hints and recommendations. Successful use in published research These columns are used in a variety of applications, including: Purification of a his-tagged ferritin protein from unclarified E. coli lysates Purification of unphosphorylated human ribosomal protein Recombinant protein purification to study the mechanism of action of antibiotic NXL101 Other Cytiva products for purifying his-tagged proteins Cytiva provides a large variety of Life Sciences solutions for IMAC protein purification. For routine his-tagged protein purification or when high resolution is a priority, consider HisTrap HP columns. Not sure which product to use? Let Cytiva help you find the right IMAC purification resin or column for your needs for your needs.