Cytiva, 17531901, HisTrap FF

Name: HisTrap FF
Brand: Cytiva
SKU: 17531901

Catalog Number: 17531901

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Category

Ion Chromatography Columns

TagsPrepacked columns Chromatography products Affinity tagged protein

CollectionsCytiva Chromatography Columns, Resins & Lab Glassware Cytiva Protein Analysis Equipment & Supplies Collection

Description

Ni Sepharose 6 Fast Flow consists of 90 µm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni ^2+ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni ^2+ ions from the matrix ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications The high flow rates made possible by the Ni Sepharose 6 Fast Flow matrix make HisTrap FF columns well suited for small scale-up. Capacity can be increased by connecting columns in series. HiTrap IMAC FF is the product of choice when charging the resin with different metal ions for optimization of purification protocols.

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