| Chromatography technique | Ion Exchange Chromatography |
|---|---|
| BioProcess resin | Yes |
| Certificate of Analysis | Yes |
| Chemical stability | Commonly used aqueous buffers, 1 M acetic acid, 1 M NaOH, 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol and 70% ethanol |
| Ion Exchanger Type | Strong cation exchanger |
| Ionic Capacity | 0.11-0.14 mmol Na + /ml medium |
| Ligand | Sulfonate group |
| Ligand Coupling Method | Dextran surface extenders |
| Matrix | Highly cross-linked agarose with dextran surface extender |
| Particle size, d 50V | ~90 μm |
| Pressure/Flow Specification | 300 kPa at 700 cm/h, 1 m diameter column, 20 cm bed height |
| Storage | 4 to 30°C, 20% Ethanol + 0.2 M Sodium Acetate |
| pH stability, CIP | 3–14 |
| pH stability, operational | 4–12 |
Answer:Capto S is a strong cation-exchange medium. Its sulfonate groups bind positively charged molecules under suitable buffer conditions.
Answer:The package contains 10 L of bulk Capto S resin intended for packing into compatible chromatography columns.
Answer:The operational pH range is 4–12. Store the resin at 4–30 °C in 20% ethanol containing 0.2 M sodium acetate; cleaning-in-place conditions differ from routine operation.
Cytiva Capto S is a high-flow strong cation-exchange resin supplied as 10 L of bulk chromatography medium for protein capture and intermediate purification. The matrix consists of highly cross-linked agarose with dextran surface extenders and negatively charged sulfonate ligands. Positively charged proteins bind to the resin under suitable pH and conductivity conditions, enabling salt- or pH-based elution from a packed process column. Its approximately 90 µm median particle size and rigid matrix are designed for high-volume throughput during downstream bioprocessing. The resin's recommended operational pH range is 4–12; cleaning-in-place stability extends from pH 3 to 14 under appropriate conditions. Capto S is supplied in 20% ethanol containing 0.2 M sodium acetate for storage at 4–30 °C. Before packing and processing, remove the storage solution and equilibrate with a suitable buffer. This is unpacked bulk resin, not a prepacked column.
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