Cytiva, 17548115, HiScreen Capto Core 700

Capto Core 700 is composed of a ligand-activated core and inactive shell. The inactive shell excludes large molecules (cut off ~ Mr 700 000 [700 kDa]) from entering the core through the pores of the shell. These larger molecules are collected in the column flowthrough while smaller impurities bind to the internalized ligands. The core of each bead is functionalized with ligands that are both hydrophobic and positively charged, resulting in a highly efficient multimodal binding of various contaminants small enough to enter the core. The multimodal ligands ensure strong binding with most impurities over a wide range of pH and salt concentrations. Bound impurities are removed from the beads by cleaning-in-place (CIP) procedures using NaOH and in most cases, a solvent. Capto Core 700 is available in various pack sizes for packing columns and in convenient, prepacked 1 mL HiTrap and 4.7 mL prepacked HiScreen formats. HiTrap and HiScreen columns can be connected in series for increased capacity and are convenient to use when developing an efficient and robust separation method.