Cytiva, 25050075, RNAspin 96 Kit

RNA extraction is the purification of RNA from biological samples. It is complicated by the ubiquitous presence of ribonuclease enzymes in cells and tissues, which can rapidly degrade RNA, since it is less stable than DNA. Besides other methods in the RNA extraction kit protocol to help counter RNA degradation the equipment used for RNA preparation is usually kept separate from other lab equipment and treated (or has been pre-treated) with chemicals that can destroy RNases, such as diethyl pyrocarbonate (DEPC). RNAspin 96 Isolation Kit can be used to reproducibly isolate total RNA from cultured cells, tissue, bacteria or yeast. It allows for the simultaneous processing of 96 samples via either centrifugation or vacuum-based protocols that can easily fit onto existing automated systems. The isolated RNA is of a high enough quality and quantity (up to 100 μg) for multiple downstream experiments, including sensitive applications such quantitative reverse transcription polymerase chain reaction (QRT-PCR), reverse transcription polymerase chain reaction (RT-PCR), gene expression profiling, primer extension, and RNase protection assays. RNAspin 96 Kit is a flexible, high throughput RNA isolation solution for total RNA purification.