Cytiva, 27084301, PreScission Protease
Catalog Number: 27084301
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| Application | Used for site-specific separation of the GST tag from proteins expressed using pGEX-6P vectors |
|---|---|
| Cleavage Buffer | 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1 mM dithiothreitol (DTT), pH 7.0 |
| Elution Buffer | 50 mM Tris-HCl, 10 mM reduced glutathione, pH 8.0 |
| Inhibitor | 100 mM ZnCl2 (< 50% inhibition); 100 µM chymostatin; 4 mM Pefabloc |
| Molecular Weight (Mr) | 46000 |
| Supplied As | 2000 units/ml (833 to 1000 units/mg) in 50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 10 mM EDTA, 1 mM DTT, and 20% glycerol. |
| Unit Definition | One unit will cleave > 90% of 100 µg of a test GST-fusion protein in 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1 mM DTT, pH 7.0 at 5°C for 16 h. |
| Vector | pGEX-6P-1, pGEX-6P-2, pGEX-6P-3 |
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