Cytiva, 28918007, PD MiniTrap G-25

Name: PD MiniTrap G-25
Brand: Cytiva
SKU: 28918007

Catalog Number: 28918007

Pricing

Send an RFQ.

Category

TagsPrepacked columns Chromatography products Desalting and buffer exchange

CollectionsCytiva Chromatography Columns, Resins & Lab Glassware Cytiva Protein Analysis Equipment & Supplies Collection

Description

Column for desalting and buffer exchange of proteins PD MiniTrap G-25 is designed for convenient desalting of biological samples. For increased flexibility, the protein desalting column can be used with either gravity or centrifugation. When using the gravity protocol, clean-up of the sample is simple. One or several columns can be connected in parallel, and a purification system is not needed. With the centrifugation protocol, the samples are run in parallel in a standard centrifuge.The eluted sample will have minimal dilution. Four adapters are included in each product pack. The adapters simplify centrifugation of these protein desalting columns. The exclusion limit is approximately M _r 5000. This ensures group separation of proteins/peptides larger than M _r 5000 from smaller molecules. Successful use in published research PD MiniTrap G-25 are used in a variety of research applications, including: Buffer exchange during in vitro assembly of catalase Removal of DTT during study of thiosulfate transfer in Metallosphaera cuprina Imidazole removal during study of disparate ligand binding sites in the human P2Y1 receptor Other Cytiva products for buffer exchange or desalting PD SpinTrap G-25 spin column for small sample quantities using a benchtop centrifuge – up to 180 µL sample volume PD MidiTrap G-25 gravity column for sample volumes up to 1 mL PD-10 Desalting gravity column for sample volumes up to 2.5 mL HiTrap Desalting columns for sample volumes up to 1.5 mL. For use with a syringe, a peristaltic pump, or a chromatography system HiPrep Desalting column for sample volumes up to 15 mL. For use with a laboratory pump or a chromatography system PD MultiTrap G-25 for high-throughput desalting and buffer exchange of multiple samples This resin is available as bulk resin

FAQs

Question: What sample volume can be processed with PD MiniTrap G-25?

Answer:Gravity-flow protocols accommodate approximately 0.1–0.5 mL, while centrifugation protocols accommodate approximately 0.2–0.5 mL.

Question: Which resin is prepacked in the desalting column?

Answer:Each PD MiniTrap G-25 column contains Sephadex G-25 Medium size-exclusion resin in a packed bed of about 2.1 mL.

Question: Can these columns be run by centrifugation instead of gravity?

Answer:Yes. Separate gravity and centrifugation protocols are supported; follow the corresponding equilibration and collection steps. The package contains 50 columns.

Application Note

Cytiva PD MiniTrap G-25 columns are single-use size-exclusion devices prepacked with Sephadex G-25 Medium resin for desalting and buffer exchange of protein samples. The chromatography matrix retains smaller molecules within its pores while larger biomolecules pass through earlier, separating proteins from salts and other low-molecular-weight compounds. Each column contains a 2.1 mL packed bed and can be used with gravity-flow or centrifugation protocols. Gravity applications handle approximately 0.1–0.5 mL of sample; centrifugation protocols handle approximately 0.2–0.5 mL. The collected elution volume and operating steps differ between these two modes, so do not interchange protocols without adjustment. The 50-column pack suits repetitive small-volume sample cleanup before downstream analysis or protein purification steps. PD MiniTrap G-25 is a desalting column rather than an affinity medium; it does not selectively bind a target protein.

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:

[email protected]

Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.