Cytiva, 28930842, ÄKTA avant 25

Name: ÄKTA avant 25
Brand: Cytiva
SKU: 28930842

Fast and secure development of scalable protein purification processes

Catalog Number: 28930842

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Category

Protein Purification Columns

TagsChromatography products Chromatography Systems

CollectionsCytiva Chromatography Columns, Resins & Lab Glassware Cytiva Protein Analysis Equipment & Supplies Collection

Description

Data file | User manual | Compare lab ÄKTA™ systems System components The figure below shows the main components of the ÄKTA™ avant system. You can configure and customize your ÄKTA™ avant system by adding optional components . Available in two versions To accommodate different flow rate/pressure specifications. The versions have the same hardware setup. System ÄKTA™ avant 25 system ÄKTA™ avant 150 system Maximum flow rate 25 mL/min 150 mL/min Maximum pressure 20 MPa 5 MPa Designed for Resin screening and method optimization Working with many samples and conditions Scaling up and robustness testing Intelligent packing of AxiChrom™ 50 columns Optimized for Smaller columns (such as HiTrap™, HiScreen™, and Tricorn™ columns) Larger columns (such as HiScale™ and AxiChrom™ columns) Note: ÄKTA™ avant systems are not designed for use in GMP-regulated applications. Typical process development tasks that you can perform with ÄKTA™ avant system: Screening chromatography resins Optimizing purification methods Intelligent column packing using preprogrammed methods Performing initial scale up Producing pure target molecules for use in preclinical studies (non-GMP) Testing process robustness Free up your time with UNICORN™ control software Control your ÄKTA™ avant system in real time with UNICORN™ software . The software is easy to use, supports productivity and data security, and enables automation. You can create methods quickly, run unattended experiments, and evaluate results efficiently. When scaling to larger ÄKTA™ systems, you’ll use the same UNICORN™ software. Streamlined design of experiments (DoE) and mechanistic modeling workflows DoE The ÄKTA™ avant system is designed for execution of DoE experimental plans. Run unattended experiments (via inlet valves, column valves, fraction collector) and execute DoE experiments at different scales. UNICORN™ DoE integrate extension enables you to automatically integrate your DoE experimental plans to UNICORN™ scouting runs. You’ll reduce the risk of errors and save time. You can do it from any commonly used DoE software available on the market. Mechanistic modeling The ÄKTA™ avant system is pre-characterized for mechanistic modeling: relevant system parameters are already included in GoSilico™ software. Furthermore, for calibration runs, data can be directly transferred from UNICORN™ software into GoSilico™ software, streamlining the modeling workflow. Chromatography resins, adsorbers, columns, and capsules We offer an extensive range of chromatography formats that can be used with the ÄKTA™ avant system. This includes: Prepacked columns, such as HiTrap™, HiScreen™, or Validation columns. Prepacked and precharacterized f (x) columns for mechanistic modeling. Empty columns, such as HiScale™ or AxiChrom™ columns. Fiber units: HiTrap Fibro™ and HiScreen Fibro™ units. Adsorber units, such as XT Acrodisc™ and Mustang™ XT capsules.

FAQs

Question: What is the normal system-pump flow range?

Answer:Approximately 0.001–25 mL/min, with a separate higher range for designated column-packing operation.

Question: What is the maximum system-pump pressure?

Answer:20 MPa (200 bar).

Question: Which purification methods can be used?

Answer:Affinity, ion-exchange, size-exclusion and hydrophobic interaction chromatography with suitable columns.

Application Note

Cytiva ÄKTA avant 25 is an automated liquid chromatography system for protein purification and process development at laboratory scale. It supports methods including affinity, ion-exchange, hydrophobic interaction and size-exclusion chromatography with compatible columns and resins. The system pumps provide a normal operating flow range of approximately 0.001–25 mL/min and a maximum rated pressure of 20 MPa. Binary gradient capability, programmable method steps, UV absorbance monitoring and automated fraction collection help maintain reproducible purification sequences. The dedicated sample pump supports controlled loading independently from the primary buffer pumps. Configure column geometry, pressure limits, sample viscosity and appropriate mixer volume for each method before operation. Prime the inlets thoroughly, degas buffers when required and perform cleaning procedures suitable for the column chemistry and process. This is a complete research-scale chromatographic instrument, not a prepacked column or software-only licence. UNICORN workstation licensing and optional system accessories require their own procurement checks.

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Unless otherwise stated, the minimum order amount is USD 1,000.
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