Cytiva, 28978243, HiScreen Blue FF

HiScreen columns are part of the process development platform available from Cytiva. The columns are prepacked with a range of BioProcess chromatography resins (resin) and designed for method optimization and parameter screening. HiScreen columns have small bed volumes (4.7 mL), reducing the cost of sample and buffer consumption. The resins used in HiScreen columns are also available in other prepacked formats and as bulk packs, for all scales of work from development and pilot studies to routine production. HiScreen Blue FF is prepacked with the BioProcess chromatography resin Blue Sepharose 6 Fast Flow. This resin has Cibacron Blue 3G covalently attached to the Sepharose 6 Fast Flow matrix by the triazine coupling method. The blue dye binds many proteins, such as albumin, interferon, lipoproteins, and blood coagulation factors. It also binds several enzymes including kinases, dehydrogenases, and most enzymes requiring adenyl-containing cofactors (e.g., NAD).