Answer:It measures pH in a compatible chromatography system's fluid monitoring assembly.
Answer:Yes. Calibrate with suitable pH buffers and verify stable readings before critical chromatography runs.
Answer:No. pH and conductivity are different measurements requiring different sensing components.
The Cytiva pH electrode measures buffer pH within a compatible ÄKTApilot or ÄKTA ready chromatography monitoring assembly. Inline pH information supports buffer preparation, method setup, and monitoring of process conditions during protein purification. Changes in pH can influence protein charge, adsorption, elution, and stability, making electrode condition an important part of system readiness. The replaceable sensor must be fitted to the correct pH monitoring unit and kept in appropriate electrode storage solution between uses. Calibrate with suitable reference buffers under the conditions required by the chromatography method, then allow the reading to stabilize before starting a run. Rinse the electrode carefully between solutions to reduce carryover and avoid mechanical damage to the sensing region. Inspect connections and seals before pressurizing the flow path. A pH electrode measures acidity; it is not a conductivity sensor or a UV detector.
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