Description
Request a demo Recent technological advancements have made single-cell omics (genomics, proteomics, metabolomics) an increasingly powerful tool for diagnosis, treatment response prediction and treatment selection for cancer. The process begins with disaggregation of a fresh or frozen sample into a single-cell suspension, followed by preparation of a single-cell next-generation sequencing (NGS) library, then data analysis. The current techniques for sample disaggregation into a single cell suspension commonly involve manual mechanical or enzymatic disruption and can result in poor yields and viability, making them unsuitable for library preparation. The VIA Extractor™ tissue disaggregator overcomes these challenges with semi-automated, gentle processing that optimizes cell viability and preserves the cell content relative to the parent sample. Read application note For omics applications, you will require the following items: VIA Extractor™ tissue disaggregator: Device for disaggregation of human and animal solid tissue or tumors. VIA Freeze™ Uno controlled-rate freezer: A liquid nitrogen-free controlled-rate freezer combining customizable freezing profiles, electronic control systems, and a conduction cooling method to optimize GMP-compliant cryopreservation. Further details can be found here . Omics clamp: Provides a secure seal after sample insertion and supports the bag during tissue dissociation. Omics pouch: Single-use, multi-compartment pouch with tubing to allow the addition of digestive enzyme solutions. Omics applicator: Open-bore sample insertion syringe to aid insertion of tissue sample into Omics pouch. To get started, you will also need: Forceps or tweezers Luer-lock 5 mL syringe Heat sealer Please note that the VIA Extractor™ tissue disaggregator needs to be placed into the VIA Freeze™ Uno controlled-rate freezer to operate. If you are not in possession of a VIA Freeze™ Uno controlled-rate freezer, the Omics bundle should be ordered. The Omics bundle includes the VIA Extractor™ tissue disaggregator, the VIA Freeze™ Uno controlled-rate freezer and the Omics clamp. * For research use only (RUO). Not for diagnostic use.
