Answer:It provides a defined sucrose response for routine SPR instrument performance checks.
Answer:No. Biacore Test Solution is 15% sucrose in HBS-EP+; BIAnormalizing Solution is a 70% glycerol solution for detector normalization.
Answer:The product is supplied as a 65 mL bottle.
Biacore Test Solution is a standard 15% (w/w) sucrose solution prepared in HBS-EP+ buffer for checking the performance of compatible Biacore surface plasmon resonance instruments. The known bulk refractive-index response provides a controlled test signal for routine system checks and maintenance verification. The 65 mL bottle is used in instrument-performance routines rather than as an analyte, ligand or calibration standard for a specific biochemical interaction. Use the relevant system-check method and buffer configuration, then examine the measured response and instrument acceptance criteria before starting binding experiments. The solution uses HBS-EP+ and differs from the discontinued BIAtest solution formulated in HBS-EP. Keep the test fluid free from contamination and apply instrument-specific maintenance instructions. Do not confuse this sucrose test fluid with the 70% glycerol normalization solution, which serves a different maintenance function.
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