Cytiva, RPN203B, Amersham Hybond-N+ (20 cm × 3 m)

Nucleic acid samples may be fixed by UV exposure or baking, although UV fixation gives better reproducibility. Hybond-N+ is not suitable for alkali blotting and fixation. All Hybond membranes are manufactured specifically for life science applications. Production runs are carefully controlled and the product exhaustively screened to ensure that only the most consistent product reaches the user. All Hybond membranes are identical on both sides. Supported nylon membranes have a high binding capacity for nucleic acid, in addition to high tensile strength. For applications requiring a high degree of sensitivity and/or reprobing these types of membranes are an ideal choice. Due to its high protein binding capacity, nylon requires extensive blocking prior to detection with antibodies to avoid high backgrounds. Consequently, nylon membranes are not recommended for use in Western blotting.