Cytiva, RPN222, Prostaglandin E₂, direct Biotrak assay

Name: Prostaglandin E₂, direct Biotrak assay
Brand: Cytiva
SKU: RPN222

Facilitate simple and rapid extraction of PGE2 from cell cultures and plasma samples

Catalog Number: RPN222

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Category

Membrane Protein Extraction Kits

TagsProtein analysis equipment and supplies Immunoassays biochemical assays accessories and reagents Detection Reagent

CollectionsCytiva Protein Analysis Equipment & Supplies Collection

Description

Lysis reagent 1 hydrolyses cell membranes to release intracellular PGE₂, or, dissociates PGE₂ from soluble receptors and interfering binding proteins present in plasma. Lysis reagent 2 sequesters the key component in lysis reagent 1 and ensures PGE₂ is free for subsequent analysis. The detergent/sequestrant complex does not interfere with antigen: antibody binding. Lysis reagent 1 is simply added to cultured cells or plasma samples, followed by a ten min incubation before assay. The antisera and PGE₂ peroxidase conjugate are reconstituted with lysis reagent 2. The assay is based on competition between unlabeled PGE₂ and a fixed quantity of peroxidase-labeled PGE₂ for a limited number of binding sites on a PGE₂-specific antibody.

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