Cytiva, RPN5661, Amersham CyDye Post-Labeling Reactive Dye Pack

Name: Amersham CyDye Post-Labeling Reactive Dye Pack
Brand: Cytiva
SKU: RPN5661

Catalog Number: RPN5661

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Category

Diagnostic ELISA Kits

TagsMolecular and Immunodiagnostics Western blotting and other genomics reagents

CollectionsCytiva Molecular & Immunodiagnostics Collection Cytiva Protein Analysis Equipment & Supplies Collection

Description

A two-step protocol to prepare cDNA probes labeled with CyDye fluorescent dyes In the first step, reverse transcriptase synthesizes first-strand cDNA, incorporating a chemically reactive nucleotide analog (amino allyl-dUTP) into the cDNA. The mRNA template is then degraded and the cDNA purified to remove free nucleotides and primers. In the second step, the purified cDNA is post-labeled with the reactive forms of Cy3 or Cy5 NHS esters that bind to the modified amino allyl-nucleotides. After a final purification, the labeled cDNA is ready for hybridization.

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