Description
Purchase orders with specific lot requests, quoted reserves and/or call off orders should be submitted through Customer Service. Fetal bovine serum (FBS) is a nutrient-rich cell culture supplement that is high in growth factors and low in antibodies, compared to non-fetal sera. Characterized FBS for cell culture HyCloneTM Characterized Fetal Bovine Serum (FBS) is an excellent alternative to more expensive defined sera, and meets the requirements of most discriminating FBS users. The final serum is assayed for provides important proteins, hormones, growth factors, metabolites, and nutrients for that support your healthy cell culture sprocess. Assay results are included in the certificate of analysis and biochemical assay list. Additional testing, including EMEA conformance testing, is also available. Bacterial endotoxin-tested Endotoxin contamination is a significant concern in research and manufacturing, especially when producing cell culture-derived products for use in humans or animals. The presence of endotoxin in cell cultures can also have adverse and unpredictable effects on cell growth, function, and phenotype that can compromise research results. Our patented closed-system blood collection method and rigorous quality control testing ensure that HyClone Characterized FBS meets industry standards of ≤ 25 EU/mL endotoxin. The final product also contains ≤ 25 mg/dL hemoglobin, which is an indicator of appropriate handling during collection and transport. Virus-tested and quality-assured for reduced contamination risk HyClone Characterized FBS is triple-filtered through serial 100 nm (0.10 μm) pore size-rated filters, which has become an industry standard for cell culture grade FBS. Before dispensing, each lot of serum is pooled using true pool technology to minimize variability from bottle to bottle. The final serum is virus-tested according to 9 CFR 113.53 to demonstrate that there are no detectable levels of specified viruses, pathogenic agents, and hemadsorbing agents. Robust sterility testing further confirms that the final product contains no detectable growth of bacteria or fungi (current USP) and no detectable mycoplasma. Post-filtration treatments for added process security Gamma-irradiated and heat-inactivated FBS options are available to further reduce the risk of adventitious agents, particularly when manufacturing products for use in humans or animals. The irradiation process is validated to deliver a minimum dose of 25 kGy. Temperature excursions happen, both in transport and on the bench. Storage condition effects on fetal bovine serum for cell culture whitepaper shows how HyClone™ serum maintain viable cell density and cell growth and stay within specification under various temperatures and timeframes.
