Cytiva, SH30859.03, HyClone CDM4HEK293 powdered medium without phenol red, L-glutamine, HEPES, sodium bicarbonate - 50 L

Cytiva HyClone CDM4HEK293 is a chemically defined, protein-free, animal-derived-component-free medium optimized for suspension culture of human embryonic kidney 293 cells. This dry-powder configuration provides the material required to prepare 50 L of complete basal medium after addition of the components specified for the established cell-culture process. It supports expansion of HEK293 cells and production of recombinant proteins and viral vectors, including adenovirus-related workflows. The formulation does not contain phenol red or L-glutamine and is designed to support high-density suspension growth under suitable cultivation conditions. The dry-powder format simplifies storage and larger-volume medium preparation for development and production environments. It is a basal cell-culture medium rather than a concentrated fed-batch supplement such as Cell Boost. Appropriate supplementation, mixing, and process conditions are determined by the HEK293 cell line and production strategy.