Description
Improve productivity and cell yield in fed-batch cultures by supplementing the cell culture medium to replace nutrients depleted during cell culture. HyClone™ Cell Boost™ 3 Supplement provides amino acids, vitamins, glucose, and trace elements to support fed-batch cultures in research and bioprocessing applications. It is available as a convenient ready-to-use liquid feed solution or as a dry powder feed to be hydrated before use. Fed-batch applications This HyClone™ cell culture supplement has been developed for recombinant protein production with various cell lines including hybridoma and myeloma cell lines. General culture recommendations Supplement 20 to 200 mL/L one or more times, starting during mid to late growth phase. This is expected to be day 3 to 4. Start the culture at a reduced volume according to anticipated feed volumes. Continue supplementation daily until the final volume is reached. It is recommended to monitor L- glutamine separately and supplement as needed. Dry powdered medium milling equivalency study This study compares pin milling of powdered HyClone™ cell culture media and supplements at two manufacturing sites. Detailed physical, chemical, and biological data, including cell growth, are provided.
