| Form | Column |
|---|---|
| Column format | Analytical |
| Phase | InertSustain C8 |
| Base Material | High Purity ES Silica Gel |
| Functional group | Octyl |
| Particle size | 3 µm |
| Length | 250 mm |
| Inner diameter | 4.0 mm |
| Mode | Reversed phase |
| Surface area | 350 m2/g |
| Pore size | 100 Å (10 nm) |
| Pore volume | 0.85 mL/g |
| End capping | Yes |
| Carbon loading | 8.0% |
| pH Range | 1-10 |
| Unit size | 1 pc. |
| Max Operating Pressure | 20 MPa(200 bar) |
| USP Code | L7 |
Answer:The analytical column is 250 mm long, has a 4.0 mm internal diameter and contains 3 µm C8-bonded particles.
Answer:Its octyl phase generally provides lower hydrophobic retention and a different selectivity profile than an octadecyl phase under comparable conditions.
Answer:This is a full-length analytical HPLC column; a compatible guard column is a separate accessory.
InertSustain C8 is an octyl-bonded reversed-phase HPLC column designed for separations that benefit from lower hydrophobic retention than a conventional C18 phase. This configuration combines 3 µm particles with a 250 mm bed length and a 4.0 mm internal diameter, supporting detailed resolution of closely eluting components in analytical methods. High-purity silica, extensive end-capping and a 100 Å pore structure contribute to controlled interactions with a broad range of small molecules. Its C8 chemistry is useful when strongly retained analytes require shorter elution times or a different selectivity balance. Choose a mobile-phase composition that preserves sufficient retention of early peaks while allowing the most hydrophobic components to elute completely. Filter samples and mobile phases to minimize particulate accumulation at the column inlet. Confirm system pressure at the selected flow rate, especially with the relatively long 3 µm bed. Maintain consistent equilibration and document changes in selectivity when transferring a method from C18 or from a shorter C8 column.
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