Merck, 1.04134, Eosin Y (yellowish) (C.I. 45380)

General description
Eosin Y (C.I. 45380) is a CERTISTAIN® dry dye for microscopy that is often used as a counterstain, vizualising proteins, connective tissues, fibers and keratin in sample material of human origin. Depending on user requirements and personal preferences, it can be used for the preparation of the commonly used aqueous or alcoholic Eosin Y staining solutions. This staining solution will mostly be used for routine staining in the hematoxylin-eosin staining process (H&E). The H&E staining process is the most frequently used staining method for histological material. Due to the hematoxylin solution, the nuclei will appear blue to dark violet in color and the Eosin Y solution will stain the cytoplasma and intercellular substances red-orange. As the Eosin Y dye belongs to the portfolio of Certistain® dyes, it is chemically analyzed according to strict specifications before release and a high staining quality, with reliable staining results is guaranteed.
Application
for microscopy Certistain®
Analysis Note
Dye content (gravimetrically): ≥ 85 %
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (water): 515 - 518 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.005 g/l; water): 1200 - 1400
TLC-Test: passes test
Loss on drying (110 °C): ≤ 8 %
Suitability for microscopy: passes test
Legal Information
CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany