Merck, 1001880004, Chromocult® RAMBACH™ Agar

General description
Chromocult® RAMBACH™ Agar is specifically designed for the differentiation of Salmonella species. The agar is chromogenic, providing two chromogenic substrates – propylene glycol and X-Gal ((5-bromo-4-chloro-3-indoyl- β-D-galactopyranoside).
Certain Salmonella species act on the propylene glycol producing acid which causes the pH indicator in the media to change color, and pinkish-red or crimson colonies appear. Additionally, other members of the family Enterobacteriaceae which are positive for the enzyme β-D-galactosidase cleave the substrate X-Gal and this results in the production of blue-green colonies.
Salmonella species that are positive for the β-D-galactosidase enzyme and can produce acid from propylene glycol appear as purple-violet colonies, while species that are capable of neither appear as yellow or colorless colonies.
Application
The Chromocult® RAMBACH™ Agar can be used for: -
The detection of Salmonella in foodstuff, water, animal feed
The differential diagnosis of Salmonella species in various samples

Analysis Note
The dehydrated medium appears as dark-green color.
The prepared medium is opaque and light pink in appearance.
The pH value at 25 degrees is in the range of 7.1-7.5.

Legal Information
CHROMOCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
Rambach is a trademark of Balel S.A.