Merck, LH20100-100G, Sephadex® LH-20
Size:100 g, Lipophilic Sephadex®
Available Catalog Numbers
Catalog Number: LH20100-100G
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Category
Pack Size100 g
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| UNSPSC Code | 13111000 |
|---|---|
| Web Name Suffix Override | 140-550 mesh |
| Web Name Override | Sephadex® LH-20 Size Exclusion Resin |
| NACRES | NB.21 |
| form | slurry |
| technique(s) | LPLC: suitable |
| matrix | Agarose |
| matrix active group | polymer |
| swelling | 1 g swells to 4 mL (water, methanol) |
| bead size | 25-100 μm |
| operating pH range | 2-13 |
| separation technique | size exclusion (SEC) |
| storage temp. | room temp |
| Quality Level | 100 |
Answer:Sephadex LH-20 supports gel permeation, partition, and adsorption separations of compounds such as lipids, steroids, vitamins, fatty acids, and plant-derived constituents.
Answer:Approximately 1 g of resin swells to about 4 mL in water or methanol; swelling varies with solvent.
Answer:The bead size is 25–100 µm, with an operating pH range of 2–13.
Sephadex LH-20 is a crosslinked, hydroxypropylated dextran chromatography resin for separating natural products and other small molecules using aqueous or organic mobile phases. Its hydrophilic and lipophilic character supports gel permeation, partition, and adsorption mechanisms, making it useful in fractionation of lipids, steroids, fatty acids, vitamins, and plant-derived compounds. The 100 g package contains gel media intended for packing a chromatography bed rather than a ready-packed column. The resin has a bead size of 25–100 µm and an operating pH range of 2–13. Its swelling behavior depends on solvent composition; approximately 1 g produces a 4 mL swollen volume in water or methanol. This dependence matters when determining the quantity required to pack a column and when changing elution conditions during method development. Equilibrate the gel in the selected mobile phase and allow swelling before evaluating the final bed volume. Sephadex LH-20 is suited to low-pressure liquid chromatography and purification workflows where solvent compatibility and the partition behavior of the target compounds determine separation conditions.
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