Merck, 10127396001, Phosphoglucose Isomerase (PGI)

Name: Phosphoglucose Isomerase (PGI)
Brand: Merck
SKU: 10127396001

Size:1 mL, glucose-6-phosphate isomerase, from yeast

Catalog Number: 10127396001

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Category

ELISA Substrates

Pack Size1 mL

TagsLabware Labware Partners Roche<sup>®</sup> Products

CollectionsMerck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Phosphoglucose Isomerase is an enzyme which possesses diagnostic potential when fractionated by cellulose acetate electrophoresis. This cytosolic enzyme plays an important role during glycolysis and glucogenesis. It acts as an tumor-secreted cytokine and autocrine motility factor. It is used as neurotrophic factor, maturation factor and as serine proteinase inhibitor.
Application
Isomerization of ketoses to aldoses.
Biochem/physiol Actions
Phosphoglucose isomerase (PGI) is specific for G6P (Km = 0.7 mM) and fructose-6-phosphate (F6P).
Physical form
Suspension in 3.2 M ammonium sulfate solution, pH approximately 6
Preparation Note
Storage conditions (working solution): A solution of PGI in 20 mM Tris, pH 8.1, containing 0.2 mg BSA/ml, is stable approx. 1 week at 2 to 8 °C.
Analysis Note
Contaminants: <0.01% F6P-K, GR, 6-PGDH and PGluM, each, <0.2% β-fructosidase
Other Notes
For life science research only. Not for use in diagnostic procedures.

Specifications

General

UNSPSC Code	12352204
Enzyme Commission (EC) Number	5.3.1.9 ( BRENDA \| IUBMB )
NACRES	NA.54
biological source	yeast
form	suspension
specific activity	~350 units/mg protein (at 25 °C with fructose-6-P as the substrate.)
packaging	pkg of 1 mL (10127396001 [2 mg])
manufacturer/tradename	Roche
optimum pH	7.0-7.6
storage temp.	2-8°C

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