Merck, 1055600001, TLC Silica gel 60 RP-18 F254S

General description
20 Aluminium sheets 5 x 7.5 cm
RP modified plates serve two purposes: act as a pilot method for HPLC and allow to choose various solvent system for special separations. Our RP-modified silica layers are well suited for many separation challenges that unmodified silica cannot overcome. These layers use aqueous solvent systems to separate extremely non-polar substances and analyze particular polar substances that can adapt to ion-pair chromatography. What’s more, RP-modified silica layers are less dependent on atmospheric humidity. Unlike unmodified silica, RP-phases do not exhibit catalytic activity. This makes them the plates of choice for unstable substance that tend to experience oxidative degradation.
Analysis Note
Specific surface area (according to BET; 5-Pt. measurement): 480 - 540 m²/g
Pore volume (N₂-isotherm): 0.74 - 0.84 ml/g
d 50 (laser diffraction, size distribution): 9.7 - 11.7 µm
Layer thickness: 150 - 200 µm
Deviation of layer thickness per plate: ≤ 30 µm
Chromatographic testings:
A) Cholesterin test
hRf-values
- cholesterin, cholesterin test:
28 - 36
B) Steroid test
- methyltestosterone, steroid test: 16 - 26
- Reichstein S, steroid test: 30 - 40
- hydrocortisone, steroid test: 42 - 52
Typical value determined on an activated sheet (120°C, 15 min)
Eluent a) aceton/ water (95/5 v/v)
Eluent b) aceton/ water (60/40 v/v)
Other Notes
Replaces: 5560-4; 5560